Peptide Storage and Stability Guidelines | Laboratory Research

Temperature Storage Requirements

Temperature control is the most critical factor in maintaining peptide stability and ensuring reproducible research outcomes. Different storage conditions serve specific purposes in peptide handling protocols.

-80°C Ultra-Low

Long-term storage (6+ months)
Maximum peptide stability
Lyophilized peptides

-20°C Standard

Medium-term storage (1-6 months)
Working stock storage
Standard laboratory protocol

2-8°C Refrigerated

Short-term working solutions
Reconstituted peptides (1-2 weeks)
Active experimental period

Environmental Control Factors

Beyond temperature, multiple environmental factors influence peptide stability. Research peptides like BPC-157 and semaglutide require careful attention to humidity, light exposure, and container materials.

Humidity Control

Maintain relative humidity below 50% for lyophilized peptides
Use desiccant packets in storage containers
Monitor humidity levels regularly
Seal containers immediately after use

Light Protection

Store in dark conditions or amber glass containers
Minimize exposure to UV and fluorescent light
Use aluminum foil wrapping when necessary
Protect during handling and preparation

Container Selection and Materials

Container materials significantly impact peptide stability and peptide purity throughout the storage period. Selection depends on storage duration, peptide properties, and analytical requirements.

Recommended Container Types:

Primary Storage:

Low-protein binding polypropylene tubes
Amber glass vials for light-sensitive peptides
Screw-cap tubes with O-ring seals

Materials to Avoid:

Standard polystyrene containers
Containers with rubber stoppers
Metal containers (oxidation risk)

Reconstitution and Working Solution Protocols

Proper reconstitution is critical for maintaining peptide stability and biological activity. Research peptides such as retatrutide require specific solvent systems and handling procedures to ensure optimal research outcomes.

Step-by-Step Reconstitution Protocol:

Preparation

Allow lyophilized peptide to equilibrate to room temperature before opening vial

Solvent Selection

Use sterile water, PBS, or peptide-specific buffer system as recommended

Reconstitution

Add solvent slowly down the side of vial, avoid direct contact with powder

Mixing

Gently swirl or use gentle vortexing; avoid vigorous agitation or foaming

Storage

Transfer to appropriate storage conditions immediately after reconstitution

Stability Testing and Monitoring

Regular stability monitoring ensures peptide integrity throughout the research period. This is particularly important for maintaining Research Use Only (RUO) standards and ensuring reproducible experimental outcomes.

Analytical Methods

HPLC analysis for purity and degradation products
Mass spectrometry for molecular integrity
Visual inspection for aggregation or precipitation
pH monitoring for solution stability

Testing Schedule

Initial characterization upon receipt
Monthly for working solutions
Quarterly for long-term storage
Before critical experiments

Documentation and Labeling Requirements

Essential Documentation Records:

Batch/lot numbers and certificates of analysis
Receipt date and initial storage conditions
Reconstitution date, volume, and concentration
Storage temperature logs and monitoring records

Stability testing results and schedules
Usage logs and aliquoting records
Expiry dates and disposal documentation
Chain of custody and personnel access logs

RUO Disclaimer

All Biovera products are for laboratory research use only (RUO).
Not for human, diagnostic, therapeutic, or veterinary use. Not evaluated or approved by the TGA or Medsafe.

Peptide Storage and Stability Guidelines for Laboratory Research